RESUMEN
The present study aims to evaluate the groundwater quality in an area characterized by significant human anthropic activities within the upper Seybouse. In order to assess the quality, a total of 20 samples were analyzed to identify the chemical and bacteriological composition of the water, its variations, and their potential impacts on the environment and human health. The results revealed concentrations of the chemical and bacteriological elements exceeding the WHO standards, with high levels of electrical conductivity (EC) (peak = 4210 µS/cm), Ca2+ (peak = 340.68 mg/L), Na+ (peak = 360 mg/L), HCO3- (peak = 287 mg/L), Cl- (peak = 542 mg/L), SO42- (peak = 687 mg/L), NO3- (pek = 65.91 mg/L), fecal coliforms (FC) (peak = 160 UFC/mL), fecal Streptococcus (FS) (peak = 43 UFC/mL), and Clostridium perfringens (CP) (peak = 29 UFC/mL). Within the basin, two different facies have been identified: Cl-SO4-Na type and Cl-SO4-Ca type. The calculated Water Quality Index (WQI) indicates that none of the groundwater samples are suitable for drinking or human consumption. The detection of pathogenic microorganisms through diverse molecular methods has revealed the existence of eight distinct species, encompassing pathogenic strains that can affect human health. Moreover, the dissolution of geologic formations can influence the water's chemistry. In this region, groundwater pollution seems to be influenced by anthropogenic and agricultural factors such as fertilizer application, irrigation practices, and the release of domestic sewage.